LetgenPro Real Time PCR Kit 100 rxn (Wilsonomyces carpophilus)

Shot-hole disease, caused by the ascomycete fungus Wilsonomyces carpophilus, is a major fungal disease of stone fruits worldwide, particularly affecting Prunus species such as apricot, peach, cherry, and almond. The pathogen causes necrotic leaf lesions that abscise, producing the characteristic “shot-hole” appearance. As these symptoms may resemble bacterial spot diseases caused by Xanthomonas species, early molecular detection is important for accurate differential diagnosis. In addition to foliar damage, W. carpophilus infects buds, twigs, and fruits, leading to premature defoliation and reduced fruit quality and market value. 

The epidemiology of W. carpophilus is closely associated with cool, wet spring conditions, during which free moisture is required for conidial germination. The fungus survives perennially in dormant tissues, particularly in infected buds and twig cankers, serving as a persistent inoculum source. Early molecular detection enables identification of the pathogen during latent or dormant stages, supporting timely integrated pest management (IPM) decisions and helping to preserve orchard productivity and yield quality.